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ProcessingProcessing

5' Biotin (Azide)

Return to Attachment Chemistry / Linkers Modifications

Biotin (Azide) is attached to the oligo sequence through a 1,2,3-triazole linkage. The 1,2,3-triazole linkage is formed from the reaction of azides and alkynes in the presence of copper (I), in the click reaction. The internal version of this modification is attached to the oligo through a dT base. Incorporation of the internal version will add a dT nucleotide at that position. To avoid adding an extra nucleotide, replace an existing T nucleotide in your sequence with the required modification.

Scientific Details
MW:486.5
Mod Code:/5BioK/
Pricing Details
ProductPricingOrder
100 nmole DNA Oligo $153.00 USD Add To Order
250 nmole DNA Oligo $191.00 USD Add To Order
1 umole DNA Oligo $284.00 USD Add To Order
2 µmole DNA Oligo $371.00 USD Add To Order
100 nmole RNA Oligo $153.00 USD Add To Order
250 nmole RNA Oligo $191.00 USD Add To Order
1 umole RNA Oligo $284.00 USD Add To Order
* Requires HPLC Purification
Structure
Structure
Purification Compatibility
Requires HPLCYes
Requires IE-HPLCNo
Allows PAGE No
Requires RNase-Free HPLC No