Molecular Biology Reagents
Documentation and Support

Buffers and Solutions

While many researchers continue to store oligonucleotides in water, resuspension in a buffered solution such as TE is recommended. DNA oligonucleotides can be damaged by prolonged incubation or storage in even mildly acidic solutions; DNA dissolved in distilled water will often have a final pH <5.0 and is at risk for depurination. RNA will degrade in alkaline conditions. Further, single-stranded nucleic acids can easily be degraded by trace contamination with a variety of nucleases that are common contaminants in a normal laboratory environment. Any solution used to resuspend and store DNA or RNA should be nuclease-free. It is recommended that stock oligo solutions be made at high concentration and stored frozen. More dilute working solutions can be made from the stocks at intervals as needed.

IDTE (1X TE Solution)

For initial resuspension and storage of DNA oligos
 

Nuclease Decontamination Solution

Irreversably inactivates nucleases—can be applied to most laboratory surfaces
 

Nuclease-Free Water

For initial resuspension and storage of single-stranded RNA oligos or making dilute solutions from stock oligos
 

Nuclease-Free Duplex Buffer

For annealing of single-stranded oligonucleotides
 

The following solutions are for resuspension and dilution of oligos. Solutions are guaranteed to be nuclease-free. Each lot is tested using our RNaseAlert® and DNaseAlert™ reagents to document the absence of any detectable nuclease activity. Individual lots are screened for endotoxins using a Limulis Abocyte Lysate (LAL) assay.

DescriptionPart #Pricing
10 x 2 ml IDTE pH 7.5 (1X TE Solution)11-01-02-02$18.00 USD
1 Liter IDTE pH 7.5 (1X TE Solution)11-05-01-05$30.00 USD
4 x 1 Liter IDTE pH 7.5 (1X TE Solution)11-05-02-03$95.00 USD
4 x 1 Liter IDTE pH 8.0 (1X TE Solution)11-05-02-04$95.00 USD
10 x 2 ml IDTE pH 8.0 (1X TE Solution)11-01-02-05$18.00 USD
1 Liter IDTE pH 8.0 (1X TE Solution)11-05-01-09$30.00 USD
300 mL IDTE pH 7.5 (1X TE Solution)11-05-01-15$18.00 USD
300 mL IDTE pH 8.0 (1X TE Solution)11-05-01-13$18.00 USD

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A 1x TE buffer for initial resuspension and storage of DNA oligos.

IDTE (10 mM Tris, pH 7.5 or 8.0, 0.1 mM EDTA)

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Many RNases and DNases are naturally found in the environment. Further, a variety of nucleases are employed in routine molecular biology methods and can accidentally contaminate lab surfaces at very high levels. In particular, RNases can be very difficult to eliminate and can cause damage to projects that require manipulations of intact RNA samples, such as functional genomics experiments, real-time quantitative PCR, microarrays, etc.

Nuclease Decontamination Solution irreversibly inactivates nucleases and can be applied to most lab surfaces to remove nuclease contamination. Just spray, rinse and let dry. Nuclease Decontamination Solution eliminates the need to bake glassware and can be applied to plastic surfaces which are difficult to sterilize.

DescriptionPart #Pricing
250 mL Nuclease Decontamination Solution11-05-01-01$35.00 USD
6 x 250 mL Nuclease Decontamination Solution11-05-03-01$165.00 USD

Nuclease Decontamination Solution

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For initial resuspension and storage of single-stranded RNA oligos. Also suitable for making dilute working solutions from stock oligos.

DescriptionPart #Pricing
1 Liter Nuclease Free Water11-05-01-04$28.00 USD
10 x 2ml Nuclease Free Water11-04-02-01$16.00 USD
4 x 1 Liter Nuclease Free Water11-05-02-02$90.00 USD
300 mL Nuclease Free Water11-05-01-14$14.00 USD

Nuclease-free Water

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For initial resuspension, annealing, and storage of duplex RNAi products.

DescriptionPart #Pricing
1 Liter Nuclease Free Duplex Buffer11-05-01-03$30.00 USD
1 Liter Nuclease Free Water11-05-01-04$28.00 USD
10 x 2 ml Nuclease Free Duplex Buffer11-01-03-01$18.00 USD
4 x 1 Liter Nuclease Free Duplex Buffer11-05-02-01$95.00 USD
300 mL Nuclease Free Duplex Buffer11-05-01-12$18.00 USD

Duplex Buffer (30 mM HEPES, pH 7.5, 100 mM Potassium Acetate)

Material Safety Data Sheets