Figure 2. Dot Blot Assay Comparing Sensitivity of Kinase- and StarFire®-Labeled Probes. Serial dilutions of target plasmid pGreen Lantern-1 were spotted on duplicate nylon membranes at 100, 10, and 1amol. An oligonucleotide specific for pGreen Lantern-1 (#R514) was labeled using the PNK and StarFire methods. One filter strip was hybridized to the StarFire-labeled probe (top), while a duplicate was hybridized to the kinase-labeled probe (bottom). Hybridization, washes, and exposures were otherwise identical.