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PrimerQuest


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Sequence Entry

Enter sequence(s) manually

Hints
  • Paste your sequence into the Textbox
  • Add up to 50 sequences in FASTA format
  • Sequence length must be greater than 80 bases
  • PrimerQuest accepts only nucleic acid bases
Sequence Name

Download sequence(s) using Genbank or Accession ID

Upload sequences in an Excel file

Choose Your Design


You'll get our 5 best assay designs for your sequence for common experiment types.

Custom Design Parameters

Set Design Parameters for...







Task Settings

Results

Off
On
Off
On
Off
On

Reaction Conditions

Primer Criteria

Primer Details

Min Opt Max

Misc Settings

id="DIVprimerMaxEndGC">

Amplicon Criteria

Amplicon Details

Min Opt Max

Custom Target Region

Primer Focus Region

Partial Design Input

Design Across Junctions

Primer Force Location Settings

Design and Analysis tools

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